Advance booking for the use of each microscope is mandatory!
INFO ❘ Nikon A1Rsi confocal system ❘ Leica Stellaris 8 confocal system
Zeiss Lightsheet 7 ❘ Zeiss Elyra 7 ❘ Zeiss Axio Observer Z1
Others ❘ Software
Only planned and trained users will have access to the microscope rooms (F-1.19, F-1.59 and G-1.140a). For reservations please send e-mail to zbmikuam@gmail.com with name, department, room, date and hours. If you plan to use the incubation system (okolab) - indicate this in your booking request. The reservation is possible up to a month in advance (15th December to 15th January and so on). Please note that student classes may be scheduled at any time and take priority.
Please plan your working time wisely.
Someone else can use the microscope!
All available microscopes are in open access mode and can be operated directly by a user after appropriate training. Each training is carried out individually. Depending on the system and user skills, training can take up to 2 - 6 hours. After the initial training, the first work should be carried out under the supervision of an employee experienced in working with the given system. Access to the microscope systems rooms is granted after the training is completed. If a user didn't use system in the last 12 months refreshing training is required.
Quota for confocal systems reservations
(Nikon A1Rsi + PicoQuant or Leica Stellaris 8 [F-1.19])
Example: User needs 5 hours slot on a confocal microscope. So user can reserve from 6:00 until 11:00, or from 14:00 until 19:00. User use 4 hours slot during standard working hours and 1 hour from outside hours.
In cases where the user really needs more than 4 hours during standard working hours (e.g. – using CO2 incubator chamber, long-term continuous data acquisition of physiological time events), it is possible to book for the whole day or longer - please consult with Bioimaging Unit staff first (AK-M / WK).
This quota system is valid only for confocal systems. Other instruments can be reserved without any restrictions.
Z-axis drift - strongly recommended to use PFS.
TIRF head not available.
Photobleaching/stimulation mode not available.
Specification of ZEISS Axio Observer Z1 microscope
Zeiss Axioskop 2 plus - upright fluorescence microscope with monochrome camera. Location: room K-1.107
Zeiss SteREO Lumar.V12 - fluorescence stereoscope with color camera. Location: room G-1.80 (key available in a drawer in room K-1.105a - on a blue lanyard)
Zeiss SteREO Lumar.V12 stereoscope booking calendar - self-service.
Leica DM4 B - upright fluorescence microscope with monochrome camera. Location: room G-1.140a (key available in a drawer in room K-1.105a - on a white lanyard)
Specification of Leica DM4 B microscope
Leica RM 2255 automated rotary microtome + Leica M50 stereoscope. Location: room G-1.140a (key available in a drawer in room K-1.105a - on a white lanyard)
In room K-1.105 there is available an extra workstation for image analysis and processing, with following software: Imaris (Bitplane, 7.6.5),
SOFTWARE
We have Bitplane Imaris floating licenses (version 7.6.5) available. Imaris is used for advanced 3D and 4D image analysis, software runs on Windows and Mac. Imaris supports many file formats: all major confocal formats including CZI, LSM, LEI, LIF, OIB, OIF, ND2, Praire, BioRad, HCImage/SimplePCI CXD. If you want to use the Imaris floating license, please contact: w.kwiatkowski@amu.edu.pl
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